Injection of rabbits with acetylcholine receptor highly purified from the electric organ of
Electrophorus electricus emulsified in complete Freund's adjuvant resulted in the production
of precipitating antibody to acetylcholine receptor. After the second injection of antigen, the
animals developed the flaccid paralysis and abnormal electromyographs characteristic of
neuromuscular blockade. Treatment with the anticholinesterases edrophonium or
neostigmine dramatically alleviated the paralysis and the fatigue seen in electromyography.