Simple assays for measuring innate interactions with fungi

AM Kerrigan, MGT de Sousa, GD Brown - Host-Fungus Interactions …, 2012 - Springer
AM Kerrigan, MGT de Sousa, GD Brown
Host-Fungus Interactions: Methods and Protocols, 2012Springer
In recent decades, there has been a steady rise in immunocompromised populations and
consequently a dramatic increase in the clinical relevance of normally non-pathogenic and
commensal fungi such as Aspergillus fumigatus and Candida albicans. Understanding how
these fungi interact with the host immune system is important for the development of
immunotherapeutic approaches. Here, we describe a number of methods which have been
developed to investigate the interactions of fungi with host leukocytes in vitro, including …
Abstract
In recent decades, there has been a steady rise in immunocompromised populations and consequently a dramatic increase in the clinical relevance of normally non-pathogenic and commensal fungi such as Aspergillus fumigatus and Candida albicans. Understanding how these fungi interact with the host immune system is important for the development of immunotherapeutic approaches. Here, we describe a number of methods which have been developed to investigate the interactions of fungi with host leukocytes in vitro, including measuring fungal binding and induction of cytokines, phagocytosis, the respiratory burst, and fungal killing.
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