A simple set of plasmids for the production of transgenic plants

M Kuroda, M Kimizu, C Mikami - Bioscience, biotechnology, and …, 2010 - Taylor & Francis
M Kuroda, M Kimizu, C Mikami
Bioscience, biotechnology, and biochemistry, 2010Taylor & Francis
A new set of plasmids for plant transgenic studies was developed. Its strong point is that
independent gene cassettes are connected within one binary vector by the restriction
endonuclease-based technique only. Using the set, two overexpressing cassettes and three
RNA interference (RNAi) cassettes were successfully introduced into rice. Our plasmid set is
useful for producing commercial transgenic plants, especially in the case of rice.
A new set of plasmids for plant transgenic studies was developed. Its strong point is that independent gene cassettes are connected within one binary vector by the restriction endonuclease-based technique only. Using the set, two overexpressing cassettes and three RNA interference (RNAi) cassettes were successfully introduced into rice. Our plasmid set is useful for producing commercial transgenic plants, especially in the case of rice.
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