In vitro karyotype evolution and cytogenetic instability in the non-tumorigenic human breast epithelial cell line HMT-3522
KV Nielsen, MW Madsen, P Briand - Cancer genetics and cytogenetics, 1994 - Elsevier
The “spontaneously” immortalized cell line HMT-3522, derived from a fibrocystic breast
lesion, is used as a model for premalignant breast epithelium. During 205 passages the
cytogenetic evolution was followed. The results were compared with our earlier results on
oncogene expression and growth factor requirements. During in vitro growth, gain and loss
of markers, loss of normal chromosomes, and duplication of the chromosome complement
could be demonstrated. The variability increased during in vitro growth, This variability …
lesion, is used as a model for premalignant breast epithelium. During 205 passages the
cytogenetic evolution was followed. The results were compared with our earlier results on
oncogene expression and growth factor requirements. During in vitro growth, gain and loss
of markers, loss of normal chromosomes, and duplication of the chromosome complement
could be demonstrated. The variability increased during in vitro growth, This variability …