[HTML][HTML] Tumor-associated carbonic anhydrase 9 spatially coordinates intracellular pH in three-dimensional multicellular growths
P Swietach, S Wigfield, P Cobden, CT Supuran… - Journal of biological …, 2008 - ASBMB
CA9 is a membrane-tethered, carbonic anhydrase (CA) enzyme, expressed mainly at the
external surface of cells, that catalyzes reversible CO 2 hydration. Expression is greatly
enhanced in many tumors, particularly in aggressive carcinomas. The functional role of CA9
in tumors is not well established. Here we show that CA9, when expressed heterologously in
cultured spheroids (0.5-mm diameter,∼ 25,000 cells) of RT112 cells (derived from bladder
carcinoma), induces a near-uniform intracellular pH (pH i) throughout the structure. Dynamic …
external surface of cells, that catalyzes reversible CO 2 hydration. Expression is greatly
enhanced in many tumors, particularly in aggressive carcinomas. The functional role of CA9
in tumors is not well established. Here we show that CA9, when expressed heterologously in
cultured spheroids (0.5-mm diameter,∼ 25,000 cells) of RT112 cells (derived from bladder
carcinoma), induces a near-uniform intracellular pH (pH i) throughout the structure. Dynamic …