Transplantation of pancreatic islets into the kidney capsule of diabetic mice
GL Szot, P Koudria, JA Bluestone - JoVE (Journal of Visualized …, 2007 - jove.com
Our protocol was developed to cleanly and easily deliver islets or cells under the kidney
capsule of diabetic or normal mice. We found that it was easier to concentrate the islets or
cells into pellets in the final delivery tubing (PE50) used to transplant the cells under the
kidney capsule. This technique provides both speed and ease while reducing any undue
stress to the cells or to the mouse. Loading: Settled, hand picked, islets or pelleted cells are
carefully aspirated off the bottom of a 1.5 mL microcentrifuge tube using a p200 pipetteman …
capsule of diabetic or normal mice. We found that it was easier to concentrate the islets or
cells into pellets in the final delivery tubing (PE50) used to transplant the cells under the
kidney capsule. This technique provides both speed and ease while reducing any undue
stress to the cells or to the mouse. Loading: Settled, hand picked, islets or pelleted cells are
carefully aspirated off the bottom of a 1.5 mL microcentrifuge tube using a p200 pipetteman …