Retroviral expression screening of oncogenes in natural killer cell leukemia
YL Choi, R Moriuchi, M Osawa, A Iwama… - Leukemia research, 2005 - Elsevier
Aggressive natural killer cell leukemia (ANKL) is an intractable malignancy that is
characterized by the outgrowth of NK cells. To identify transforming genes in ANKL, we
constructed a retroviral cDNA expression library from an ANKL cell line KHYG-1. Infection of
3T3 cells with recombinant retroviruses yielded 33 transformed foci. Nucleotide sequencing
of the DNA inserts recovered from these foci revealed that 31 of them encoded KRAS2 with
a glycine-to-alanine mutation at codon 12. Mutation-specific PCR analysis indicated that the …
characterized by the outgrowth of NK cells. To identify transforming genes in ANKL, we
constructed a retroviral cDNA expression library from an ANKL cell line KHYG-1. Infection of
3T3 cells with recombinant retroviruses yielded 33 transformed foci. Nucleotide sequencing
of the DNA inserts recovered from these foci revealed that 31 of them encoded KRAS2 with
a glycine-to-alanine mutation at codon 12. Mutation-specific PCR analysis indicated that the …