In vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres
GS Im, L Lai, Z Liu, Y Hao, D Wax, A Bonk, RS Prather - Theriogenology, 2004 - Elsevier
This study investigated the effect of culture media and gas atmospheres on the development
of porcine nuclear transfer embryos. Oocytes derived from a local abattoir were matured for
42–44h and enucleated. Fetal fibroblasts were prepared from a Day 35 porcine fetus.
Confluent stage fetal fibroblasts were introduced into the perivitelline space of enucleated
oocytes. Fusion and activation were induced simultaneously with two direct current (1.2
kV/cm for 30μs) in 0.3 M mannitol medium. For parthenogenetic activation, the same pulses …
of porcine nuclear transfer embryos. Oocytes derived from a local abattoir were matured for
42–44h and enucleated. Fetal fibroblasts were prepared from a Day 35 porcine fetus.
Confluent stage fetal fibroblasts were introduced into the perivitelline space of enucleated
oocytes. Fusion and activation were induced simultaneously with two direct current (1.2
kV/cm for 30μs) in 0.3 M mannitol medium. For parthenogenetic activation, the same pulses …