Sphingosine kinase isoforms regulate oxaliplatin sensitivity of human colon cancer cells through ceramide accumulation and Akt activation
S Nemoto, M Nakamura, Y Osawa, S Kono… - Journal of Biological …, 2009 - ASBMB
The relationship between sphingosine kinase (SPHK), cellular ceramide concentration and
chemosensitivity was investigated in human colon cancer cell lines. Among nine colon
cancer cell lines, SPHK1 and SPHK2 activity and protein expression was highest in RKO
cells and lowest in HCT116 cells. A viability assay revealed that HCT116 cells were
sensitive to the effects of oxaliplatin (l-OHP), whereas RKO cells were resistant to those of l-
OHP. Treatment with 5μg/ml l-OHP induced a marked time-dependent increase in various …
chemosensitivity was investigated in human colon cancer cell lines. Among nine colon
cancer cell lines, SPHK1 and SPHK2 activity and protein expression was highest in RKO
cells and lowest in HCT116 cells. A viability assay revealed that HCT116 cells were
sensitive to the effects of oxaliplatin (l-OHP), whereas RKO cells were resistant to those of l-
OHP. Treatment with 5μg/ml l-OHP induced a marked time-dependent increase in various …
