IL-23 production by cosecretion of endogenous p19 and transgenic p40 in keratin 14/p40 transgenic mice: evidence for enhanced cutaneous immunity

T Kopp, P Lenz, C Bello-Fernandez… - The Journal of …, 2003 - journals.aai.org
T Kopp, P Lenz, C Bello-Fernandez, RA Kastelein, TS Kupper, G Stingl
The Journal of Immunology, 2003journals.aai.org
Abstract p40, the common subunit of the proinflammatory cytokines IL-12 and IL-23, is
produced by resident skin cells. Whereas the in vivo effects of IL-12 are well established,
little is known about the role of IL-23 in cutaneous immune responses. In this study we show
that p40 transgenic (TG) mice constitutively produce IL-23 (p19/p40), but not IL-12
(p35/p40), in basal keratinocytes by cosecretion of TG p40 with endogenous p19. Repeated
injections of rIL-23 in littermate (LM) mice result in an inflammatory skin disease similar to …
Abstract
p40, the common subunit of the proinflammatory cytokines IL-12 and IL-23, is produced by resident skin cells. Whereas the in vivo effects of IL-12 are well established, little is known about the role of IL-23 in cutaneous immune responses. In this study we show that p40 transgenic (TG) mice constitutively produce IL-23 (p19/p40), but not IL-12 (p35/p40), in basal keratinocytes by cosecretion of TG p40 with endogenous p19. Repeated injections of rIL-23 in littermate (LM) mice result in an inflammatory skin disease similar to that of p40 TG mice, confirming the proinflammatory activity of IL-23. Furthermore, IL-23 secretion by p40 TG keratinocytes induces elevated numbers of Langerhans cells (LC) with a marked up-regulation of costimulatory molecules, indicating advanced maturation of keratin 14 (K14)/p40 LC when compared with LM LC. At the functional level, freshly isolated K14/p40 LC greatly exceeded LC from LM animals in their capacity to stimulate allogeneic T cell proliferation. To assess whether IL-23 regulates cutaneous immune responses in vivo, we used an allogeneic skin transplantation model. Full thickness skin grafts from K14/p40 donors (H-2 q) transplanted across a MHC class I and class II barrier onto BALB/c (H-2 d) recipients were rejected in a significantly accelerated fashion (mean survival time: 8.8 days) when compared with skin grafts from non-TG LM (H-2 q)(mean survival time: 10.7 days, p< 0.01). Based on these results we propose that IL-23-induced changes of LC may be an important mechanism in directing the outcome of cutaneous immune responses.
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