[HTML][HTML] A Pancreatic β-Cell-specific Enhancer in the HumanPDX-1 Gene Is Regulated by Hepatocyte Nuclear Factor 3β (HNF-3β), HNF-1α, and SPs Transcription …
E Ben-Shushan, S Marshak, M Shoshkes… - Journal of Biological …, 2001 - ASBMB
The PDX-1 transcription factor plays a key role in pancreas development. Although
expressed in all cells at the early stages, in the adult it is mainly restricted to the β-cell. To
characterize the regulatory elements and potential transcription factors necessary for human
PDX-1 gene expression in β-cells, we constructed a series of 5′ and 3′ deletion
fragments of the 5′-flanking region of the gene, fused to the luciferase reporter gene. In this
report, we identify by transient transfections in β-and non-β-cells a novel β-cell-specific distal …
expressed in all cells at the early stages, in the adult it is mainly restricted to the β-cell. To
characterize the regulatory elements and potential transcription factors necessary for human
PDX-1 gene expression in β-cells, we constructed a series of 5′ and 3′ deletion
fragments of the 5′-flanking region of the gene, fused to the luciferase reporter gene. In this
report, we identify by transient transfections in β-and non-β-cells a novel β-cell-specific distal …