[HTML][HTML] The farnesoid X receptor induces very low density lipoprotein receptor gene expression

A Sirvent, T Claudel, G Martin, J Brozek, V Kosykh… - FEBS letters, 2004 - Elsevier
A Sirvent, T Claudel, G Martin, J Brozek, V Kosykh, R Darteil, DW Hum, JC Fruchart, B Staels
FEBS letters, 2004Elsevier
The farnesoid X receptor (FXR) is a nuclear receptor activated by bile acids (BAs). In
response to ligand-binding, FXR regulates many genes involved in BA, lipid, and lipoprotein
metabolism. To identify new FXR target genes, microarray technology was used to profile
total RNA extracted from HepG2 cells treated with the natural FXR agonist
chenodeoxycholic acid (CDCA). Interestingly, a significant increase of transcript level of the
very low density lipoprotein receptor (VLDLR) was observed. Our data, resulting from …
The farnesoid X receptor (FXR) is a nuclear receptor activated by bile acids (BAs). In response to ligand-binding, FXR regulates many genes involved in BA, lipid, and lipoprotein metabolism. To identify new FXR target genes, microarray technology was used to profile total RNA extracted from HepG2 cells treated with the natural FXR agonist chenodeoxycholic acid (CDCA). Interestingly, a significant increase of transcript level of the very low density lipoprotein receptor (VLDLR) was observed. Our data, resulting from selective FXR activation, FXR RNA silencing and FXR-deficient mice, clearly demonstrate that BAs up-regulate VLDLR transcript levels via a FXR-dependent mechanism in vitro in human and in vivo in mouse liver cells.
Elsevier