Expression of insulin-responsive glucose transporter GLUT4 mRNA in the rat brain and spinal cord: an in situ hybridization study
S El Messari, A Aı̈t-Ikhlef, DH Ambroise… - Journal of chemical …, 2002 - Elsevier
Following a previous immunocytochemical study of GLUT4 in the rat brain and spinal cord
(J. Comp. Neurol. 399 (1998) 492), we now report the distribution and cellular expression of
GLUT4 mRNA in the CNS using reverse transcription-polymerase chain reaction and non-
radioactive in situ hybridization (ISH). The former technique demonstrated the expression of
GLUT4 in the different regions examined while ISH with a specific riboprobe allowed the
anatomical localization of GLUT4 mRNA. A strong hybridization signal was detected in the …
(J. Comp. Neurol. 399 (1998) 492), we now report the distribution and cellular expression of
GLUT4 mRNA in the CNS using reverse transcription-polymerase chain reaction and non-
radioactive in situ hybridization (ISH). The former technique demonstrated the expression of
GLUT4 in the different regions examined while ISH with a specific riboprobe allowed the
anatomical localization of GLUT4 mRNA. A strong hybridization signal was detected in the …