Tumor growth is angiogenesis dependent. As a consequence, strategies aimed at disrupting
this mechanism are heavily investigated. Several angiogenesis assays are used to directly
compare the efficacy of anti-angiogenic compounds. However, objective assessment of new
vascular growth has been difficult to achieve. The aim of this study was to test and develop a
computer-assisted image analysis method that would give an unbiased quantification of the
microvessel density. Human tumors were grown in athymic mice and tumor biopsies were …