N-acetyl-p-benzoquinone imine-induced changes in the energy metabolism in hepatocytes
BS Andersson, M Rundgren, SD Nelson… - Chemico-biological …, 1990 - Elsevier
The effect of N-acetyl-p-benzoquinone imine (NAPQI), a reactive metabolite of
acetaminophen, on the energy metabolism in isolated hepatocytes was investigated.
Incubation of cells with NAPQI (400 μM) resulted in an immediate uptake into the
mitochondria, followed by both reduction and glutathione conjugation of the quinone imine.
These reactions were extremely rapid and were associated with depletion of the
mitochondrial ATP content (> 80% depletion after 1 min exposure). The loss of ATP was …
acetaminophen, on the energy metabolism in isolated hepatocytes was investigated.
Incubation of cells with NAPQI (400 μM) resulted in an immediate uptake into the
mitochondria, followed by both reduction and glutathione conjugation of the quinone imine.
These reactions were extremely rapid and were associated with depletion of the
mitochondrial ATP content (> 80% depletion after 1 min exposure). The loss of ATP was …