Genetic instability and diminished differentiation capacity in long-term cultured mouse neurosphere cells
V Vukicevic, A Jauch, TC Dinger, L Gebauer… - Mechanisms of ageing …, 2010 - Elsevier
The potential use of neural stem cells in basic research, drug testing and for development of
therapeutic strategies requires large scale in vitro amplification, increasing the probability of
genetic instability and transformation. Little is known, however, about potential correlations
between long-term culture of neural stem and progenitor cells (NSPCs), changed
differentiation and self-renewal capacities, and the occurrence of chromosomal instability.
This study investigates the effect of extended culture time on self-renewal, differentiation …
therapeutic strategies requires large scale in vitro amplification, increasing the probability of
genetic instability and transformation. Little is known, however, about potential correlations
between long-term culture of neural stem and progenitor cells (NSPCs), changed
differentiation and self-renewal capacities, and the occurrence of chromosomal instability.
This study investigates the effect of extended culture time on self-renewal, differentiation …