[HTML][HTML] Unconjugated bilirubin exhibits spontaneous diffusion through model lipid bilayers and native hepatocyte membranes
SD Zucker, W Goessling, AG Hoppin - Journal of Biological Chemistry, 1999 - ASBMB
The liver is responsible for the clearance and metabolism of unconjugated bilirubin, the
hydrophobic end-product of heme catabolism. Although several putative bilirubin
transporters have been described, it has been alternatively proposed that bilirubin enters the
hepatocyte by passive diffusion through the plasma membrane. In order to elucidate the
mechanism of bilirubin uptake, we measured the rate of bilirubin transmembrane diffusion
(flip-flop) using stopped-flow fluorescence techniques. Unconjugated bilirubin rapidly …
hydrophobic end-product of heme catabolism. Although several putative bilirubin
transporters have been described, it has been alternatively proposed that bilirubin enters the
hepatocyte by passive diffusion through the plasma membrane. In order to elucidate the
mechanism of bilirubin uptake, we measured the rate of bilirubin transmembrane diffusion
(flip-flop) using stopped-flow fluorescence techniques. Unconjugated bilirubin rapidly …