[HTML][HTML] Nutrient deprivation induces neuronal autophagy and implicates reduced insulin signaling in neuroprotective autophagy activation
JE Young, RA Martinez, AR La Spada - Journal of Biological Chemistry, 2009 - ASBMB
Although autophagy maintains normal neural function by degrading misfolded proteins, little
is known about how neurons activate this integral response. Furthermore, classical methods
of autophagy induction used with nonneural cells, such as starvation, simply result in neuron
death. To study neuronal autophagy, we cultured primary cortical neurons from transgenic
mice that ubiquitously express green fluorescent protein-tagged LC3 and monitored LC3-I to
LC3-II conversion by immunohistochemistry and immunoblotting. Evaluation of different …
is known about how neurons activate this integral response. Furthermore, classical methods
of autophagy induction used with nonneural cells, such as starvation, simply result in neuron
death. To study neuronal autophagy, we cultured primary cortical neurons from transgenic
mice that ubiquitously express green fluorescent protein-tagged LC3 and monitored LC3-I to
LC3-II conversion by immunohistochemistry and immunoblotting. Evaluation of different …