Allergens in bee venom: I. Separation and identification of the major allergens
DR Hoffman, WH Shipman - Journal of Allergy and Clinical Immunology, 1976 - Elsevier
Honeybee venom was separated into seven fractions by gel filtration on Sephadex G-75.
Allergenic activities of these fractions were assessed by the paper disc radioallergosorbent
test (RAST) with a panel of sera from 24 individuals who had systemic reactions to bee
stings, 7 who had large local reactions, and 10 control subjects who had reactions of 5 cm or
less following bee stings. Three fractions were identified by enzyme or direct hemolytic
activity. Twenty-nine of 31 sera from patients having either systemic or large local reactions …
Allergenic activities of these fractions were assessed by the paper disc radioallergosorbent
test (RAST) with a panel of sera from 24 individuals who had systemic reactions to bee
stings, 7 who had large local reactions, and 10 control subjects who had reactions of 5 cm or
less following bee stings. Three fractions were identified by enzyme or direct hemolytic
activity. Twenty-nine of 31 sera from patients having either systemic or large local reactions …