[HTML][HTML] Rapid Ca2+ influx and diacylglycerol synthesis in growth hormone-mediated islet β-cell mitogenesis
Å Sjöholm, Q Zhang, N Welsh, A Hansson… - Journal of Biological …, 2000 - ASBMB
Growth hormone (GH) is an important mitogenic stimulus for the insulin-producing β-cell. We
investigated the effects of GH on Ca 2+ handling and diacylglycerol (DAG) and cAMP
formation in the β-cell. GH elicited a rapid increase in the cytoplasmic free [Ca 2+], which
required extracellular Ca 2+ and was also blocked by pertussis toxin or protein kinase C
(PKC) inhibition. GH also elevated islet DAG content, which should lead to PKC activation.
Pertussis toxin and PKC inhibitors obliterated the mitogenicity of GH, suggesting …
investigated the effects of GH on Ca 2+ handling and diacylglycerol (DAG) and cAMP
formation in the β-cell. GH elicited a rapid increase in the cytoplasmic free [Ca 2+], which
required extracellular Ca 2+ and was also blocked by pertussis toxin or protein kinase C
(PKC) inhibition. GH also elevated islet DAG content, which should lead to PKC activation.
Pertussis toxin and PKC inhibitors obliterated the mitogenicity of GH, suggesting …