Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays

C Pfleger, N Schloot, F ter Veld - Journal of immunological methods, 2008 - Elsevier
INTRODUCTION: In the Luminex multiplexed immunoassay system, complex samples such
as human serum are diluted to minimize disturbing matrix effects with a specific diluent. This
diluent has to imitate the sample matrix to allow interpolation and has to provide optimal
cytokine–antibody binding for all cytokines. Because diluents influence multiplex
immunoassay results, this paper explores several methods to determine the quality of a
chosen diluent. MATERIALS AND METHODS: Two commercially available diluents, DY997 …