Identification of intermediates after inhibition of cholesterol synthesis by aminotriazole treatment in vivo
F Hashimoto, H Hayashi - Biochimica et Biophysica Acta (BBA)-Lipids and …, 1991 - Elsevier
Cholesterol synthesis from mevalonate is inhibited by aminotriazole treatment in vivo. We
tried to identify intermediates accumulated in liver of aminotriazole-treated rats. At 6 h after
the aminotriazole treatment, the liver was excised. Sterols were extracted from it, and
subjected to capillary gas-liquid chromatography, high-performance liquid chromatography,
gas-liquid chromatography linked to mass spectrometry and gas-liquid chromatography
linked to Fourier-transform infrared spectrometry. It was found that 4α-methyl-5α-cholest-7 …
tried to identify intermediates accumulated in liver of aminotriazole-treated rats. At 6 h after
the aminotriazole treatment, the liver was excised. Sterols were extracted from it, and
subjected to capillary gas-liquid chromatography, high-performance liquid chromatography,
gas-liquid chromatography linked to mass spectrometry and gas-liquid chromatography
linked to Fourier-transform infrared spectrometry. It was found that 4α-methyl-5α-cholest-7 …