[HTML][HTML] The sulfate groups of chondroitin sulfate-and heparan sulfate-containing proteoglycans in neutrophil plasma membranes are novel binding sites for human …
EJ Campbell, CA Owen - Journal of biological chemistry, 2007 - ASBMB
Human leukocyte elastase (HLE) and cathepsin G (CG) are expressed at high levels on the
surface of activated human neutrophils (PMN) in catalytically active but inhibitor-resistant
forms having the potential to contribute to tissue injury. Herein we have investigated the
mechanisms by which HLE and CG bind to PMN plasma membranes. 125 I-Labeled HLE
and CG bind to PMN at 0° C in a saturable and reversible manner (KD= 5.38 and 4.36× 10-7
m and 11.5 and 8.1× 10 6 binding sites/cell, respectively). Incubation of PMN with …
surface of activated human neutrophils (PMN) in catalytically active but inhibitor-resistant
forms having the potential to contribute to tissue injury. Herein we have investigated the
mechanisms by which HLE and CG bind to PMN plasma membranes. 125 I-Labeled HLE
and CG bind to PMN at 0° C in a saturable and reversible manner (KD= 5.38 and 4.36× 10-7
m and 11.5 and 8.1× 10 6 binding sites/cell, respectively). Incubation of PMN with …