Measles infection in infants is associated with severe complications, and secondary infections are attributed to generalized immunosuppression. Measles binding to its monocyte receptor down-regulates IL-12 which is expected to diminish Th1-like cytokine responses, including IFN-γ. Whether young infants can be immunized effectively against measles is an important public health issue. We evaluated Ag-specific IL-12, IFN-γ, and T cell responses of infants at 6 (n= 60), 9 (n= 46), or 12 mo (n= 56) of age and 29 vaccinated adults. IL-12 and IFN-γ release by PBMC stimulated with measles Ag increased significantly after measles immunization in infants. IL-12 and IFN-γ concentrations were equivalent in younger and older infants, but IL-12 concentrations were significantly lower in infants than in adults (p= 0.04). IL-12 production by monocytes was down-regulated by measles; the addition of recombinant human IL-12 enhanced IFN-γ production by PBMC stimulated with measles Ag, but infant T cells released significantly less IFN-γ than adult T cells under this condition. Of particular interest, the presence of passive Abs to measles had no effect on the specific T cell proliferation or IFN-γ production after measles stimulation. Cellular immunity to measles infection and vaccination may be limited in infants compared with adults as a result of less effective IFN-γ and IL-12 production in response to measles Ags. These effects were not exaggerated in younger infants compared with effects in infants who were immunized at 12 mo. In summary, infant T cells were primed with measles Ag despite the presence of passive Abs, but their adaptive immune responses were limited compared with those of adults.