The dissociation of the human vitamin A-transporting protein complex into free prealbumin
and free retinol-binding protein (RBP) was studied by gel chromatography under varying
conditions of pH and ionic strength. The interaction between the two proteins was
diminished at a low ionic strength and complete dissociation seemed to occur at an ionic
strength of 0.002. Variations in pH did not appear to alter seriously the affinity between
prealbumin and RBP as the prealbumin-RBP complex was the predominant component in …