A human hepatocellular in vitro model to investigate steatosis

MJ Gómez-Lechón, MT Donato… - Chemico-biological …, 2007 - Elsevier
The present study was designed to define an experimental model of hepatocellular steatosis
with a fat overaccumulation profile in which the metabolic and cytotoxic/apoptotic effects
could be separated. This was accomplished by defining the experimental conditions of lipid
exposure that lead to significant intracellular fat accumulation in the absence of overt
cytotoxicity, therefore allowing to differentiate between cytotoxic and apoptotic effects.
Palmitic (C16: 0) and oleic (C18: 1) acids are the most abundant fatty acids (FFAs) in liver …