[HTML][HTML] A Novel Ubiquitous Form of Munc-18 Interacts with Multiple Syntaxins.: USE OF THE YEAST TWO-HYBRID SYSTEM TO STUDY INTERACTIONS BETWEEN …
Y Hata, TC Südhof - Journal of Biological Chemistry, 1995 - ASBMB
Munc-18-1 is a 67-kDa neuronal protein that binds tightly to syntaxin 1 and functions in
synaptic vesicle exocytosis (Hata, Y., Slaughter, CA, and Südhof, TC (1993a) Nature 366,
347-351). We have now characterized a new Munc-18 isoform, Munc-18-2, that exhibits
63% amino acid sequence identity with Munc-18-1. Munc-18-2 is expressed in most tissues,
whereas Munc-18-1 is primarily expressed in brain. Using recombinant Munc-18-1 and
Munc-18-2 produced in COS cells, we show that both forms of Munc-18 bind tightly to …
synaptic vesicle exocytosis (Hata, Y., Slaughter, CA, and Südhof, TC (1993a) Nature 366,
347-351). We have now characterized a new Munc-18 isoform, Munc-18-2, that exhibits
63% amino acid sequence identity with Munc-18-1. Munc-18-2 is expressed in most tissues,
whereas Munc-18-1 is primarily expressed in brain. Using recombinant Munc-18-1 and
Munc-18-2 produced in COS cells, we show that both forms of Munc-18 bind tightly to …
