Activation of NFAT-dependent gene expression by Nef: conservation among divergent Nef alleles, dependence on SH3 binding and membrane association, and …
A Manninen, P Huotari, M Hiipakka… - Journal of …, 2001 - Am Soc Microbiol
Journal of virology, 2001•Am Soc Microbiol
Here we show that the potential to regulate NFAT is a conserved property of different Nef
alleles and that Nef residues involved in membrane targeting and SH3 binding are critical
for this function. Cotransfection of an activated protein kinase C-θ (PKC-θ) with Nef
implicated PKC-θ as a possible physiological cofactor of Nef in promoting NFAT-dependent
gene expression and T-cell activation.
alleles and that Nef residues involved in membrane targeting and SH3 binding are critical
for this function. Cotransfection of an activated protein kinase C-θ (PKC-θ) with Nef
implicated PKC-θ as a possible physiological cofactor of Nef in promoting NFAT-dependent
gene expression and T-cell activation.
Abstract
Here we show that the potential to regulate NFAT is a conserved property of different Nef alleles and that Nef residues involved in membrane targeting and SH3 binding are critical for this function. Cotransfection of an activated protein kinase C-θ (PKC-θ) with Nef implicated PKC-θ as a possible physiological cofactor of Nef in promoting NFAT-dependent gene expression and T-cell activation.
American Society for Microbiology