Generation of high quantities of viral and tumor-specific human CD4+ and CD8+ T-cell clones using peptide pulsed mature dendritic cells

JF Fonteneau, M Larsson, S Somersan… - Journal of …, 2001 - Elsevier
CD4+ and CD8+ T cells are key components of immune response against tumors and
viruses. Many techniques have been used to clone and expand these cells in vitro for
purposes of immunotherapy. Here, we describe an improved method to obtain large
quantities of tumor and virus-specific human CD4+ and CD8+ T-cell clones. T cells derived
from peripheral blood mononuclear cells (PBMCs) of healthy donors were stimulated
several times by peptide pulsed monocyte-derived mature dendritic cells (DCs) in the …