Expression and purification of the angiogenesis inhibitor 16-kDa prolactin fragment from insect cells
M Galfione, W Luo, J Kim, D Hawke… - Protein expression and …, 2003 - Elsevier
The 16-kDa fragment of prolactin (16-kDa PRL), derived from proteolytic cleavage of 23-kDa
PRL, was shown to have antiangiogenic activity. Previous studies have shown that
recombinant 16-kDa PRL produced from bacteria often contained endotoxins, which are
cytotoxic to endothelial cells, and varied in its biological activity due to changes in its
refolding from inclusion bodies. These problems limited the use of recombinant 16-kDa PRL.
To improve the generation of recombinant 16-kDa PRL, we expressed 16-kDa PRL in Sf9 …
PRL, was shown to have antiangiogenic activity. Previous studies have shown that
recombinant 16-kDa PRL produced from bacteria often contained endotoxins, which are
cytotoxic to endothelial cells, and varied in its biological activity due to changes in its
refolding from inclusion bodies. These problems limited the use of recombinant 16-kDa PRL.
To improve the generation of recombinant 16-kDa PRL, we expressed 16-kDa PRL in Sf9 …