[HTML][HTML] Protein phosphatases 2A and 1 interact with occludin and negatively regulate the assembly of tight junctions in the CACO-2 cell monolayer
Occludin is hyperphosphorylated on Ser and Thr residues in intact epithelial tight junction
(TJ); however, the role of this phosphorylation in the assembly of TJ is unclear. The influence
of protein phosphatases PP2A and PP1 on the assembly of TJ and phosphorylation of
occludin was evaluated in Caco-2 cells. Protein phosphatase inhibitors and reduced
expression of PP2A-Cα and PP1α accelerated the calcium-induced increase in
transepithelial electrical resistance and barrier to inulin permeability and also enhanced the …
(TJ); however, the role of this phosphorylation in the assembly of TJ is unclear. The influence
of protein phosphatases PP2A and PP1 on the assembly of TJ and phosphorylation of
occludin was evaluated in Caco-2 cells. Protein phosphatase inhibitors and reduced
expression of PP2A-Cα and PP1α accelerated the calcium-induced increase in
transepithelial electrical resistance and barrier to inulin permeability and also enhanced the …