[HTML][HTML] 27-hydroxycholesterol: production rates in normal human subjects
WC Duane, NB Javitt - Journal of lipid research, 1999 - ASBMB
We attempted to quantitate production of bile acid via the 27-hydroxylation pathway in six
human subjects. After bolus intravenous injection of known amounts of [24-14 C] cholic acid
and [24-14 C] chenodeoxycholic acid, each subject underwent a constant intravenous
infusion of a mixture of [22, 23-3 H]-27-hydroxycholesterol and [2 H]-27-hydroxycholesterol
for 6–10 h. Production rate of 27-hydroxycholesterol was calculated from the infusion rate of
[2 H]-27-hydroxycholesterol and the serum ratio of deuterated/protium 27 …
human subjects. After bolus intravenous injection of known amounts of [24-14 C] cholic acid
and [24-14 C] chenodeoxycholic acid, each subject underwent a constant intravenous
infusion of a mixture of [22, 23-3 H]-27-hydroxycholesterol and [2 H]-27-hydroxycholesterol
for 6–10 h. Production rate of 27-hydroxycholesterol was calculated from the infusion rate of
[2 H]-27-hydroxycholesterol and the serum ratio of deuterated/protium 27 …