[HTML][HTML] Mek1 phosphorylation site mutants activate Raf-1 in NIH 3T3 cells
A Alessandrini, H Greulich, W Huang… - Journal of Biological …, 1996 - ASBMB
MAP (mitogen-activated protein) kinases are activated by a family of dual specificity kinases
called Meks (MAP kinase/Erk kinase). Mek1 can be activated by Raf by phosphorylation on
serine 218 and serine 222. Mutation of these sites to acidic residues leads to constitutively
active Mek1 in some cases. When fibroblast lines were infected with high titer retroviral
stocks carrying these Mek1 genes, the resultant transformation and morphological changes
correlated with the kinase activity of the respective Mek1 enzymes. Although [Asp 218]-and …
called Meks (MAP kinase/Erk kinase). Mek1 can be activated by Raf by phosphorylation on
serine 218 and serine 222. Mutation of these sites to acidic residues leads to constitutively
active Mek1 in some cases. When fibroblast lines were infected with high titer retroviral
stocks carrying these Mek1 genes, the resultant transformation and morphological changes
correlated with the kinase activity of the respective Mek1 enzymes. Although [Asp 218]-and …