[HTML][HTML] Presenilin-1 maintains a nine-transmembrane topology throughout the secretory pathway
D Spasic, A Tolia, K Dillen, V Baert… - Journal of Biological …, 2006 - ASBMB
Presenilin-1 is a polytopic membrane protein that assembles with nicastrin, PEN-2, and APH-
1 into an active γ-secretase complex required for intramembrane proteolysis of type I
transmembrane proteins. Although essential for a correct understanding of structure-function
relationships, its exact topology remains an issue of strong controversy. We revisited
presenilin-1 topology by inserting glycosylation consensus sequences in human PS1 and
expressing the obtained mutants in a presenilin-1 and 2 knock-out background. Based on …
1 into an active γ-secretase complex required for intramembrane proteolysis of type I
transmembrane proteins. Although essential for a correct understanding of structure-function
relationships, its exact topology remains an issue of strong controversy. We revisited
presenilin-1 topology by inserting glycosylation consensus sequences in human PS1 and
expressing the obtained mutants in a presenilin-1 and 2 knock-out background. Based on …