Enumerating antigen-specific T-cell responses in peripheral blood: a comparison of peptide MHC Tetramer, ELISpot, and intracellular cytokine analysis
AC Hobeika, MA Morse, T Osada… - Journal of …, 2005 - journals.lww.com
Detection of the circulating antigen-specific T-cell response to immunization is an important
biologic end point in clinical trials of cancer vaccines. Typically employed assays are
peptide MHC tetramer, ELISpot, and intracellular cytokine analysis. Although there is no
agreement on the definition of a positive response in these assays, many groups have
chosen a number of T cells greater than 2 standard deviations above the mean of the
negative controls. The authors wished to determine how well this cutoff performed for each …
biologic end point in clinical trials of cancer vaccines. Typically employed assays are
peptide MHC tetramer, ELISpot, and intracellular cytokine analysis. Although there is no
agreement on the definition of a positive response in these assays, many groups have
chosen a number of T cells greater than 2 standard deviations above the mean of the
negative controls. The authors wished to determine how well this cutoff performed for each …