Molecular characterization and mutational analysis of the human B17 subunit of the mitochondrial respiratory chain complex I

J Smeitink, J Loeffen, R Smeets, R Triepels… - Human genetics, 1998 - Springer
J Smeitink, J Loeffen, R Smeets, R Triepels, W Ruitenbeek, F Trijbels, L van den Heuvel
Human genetics, 1998Springer
Bovine NADH: ubiquinone oxidoreductase (complex I) of the mitochondrial respiratory chain
consists of about 36 nuclear-encoded subunits. We review the current knowledge of the 15
human complex I subunits cloned so far, and report the 598-bp cDNA sequence, the
chromosomal localization and the tissue expression of an additional subunit, the B17
subunit. The cDNA open reading frame of B17 comprises 387 bp and encodes a protein of
128 amino acids (calculated M r 15.5 kDa). There is 82.7% and 78.1% homology …
Abstract
Bovine NADH:ubiquinone oxidoreductase (complex I) of the mitochondrial respiratory chain consists of about 36 nuclear-encoded subunits. We review the current knowledge of the 15 human complex I subunits cloned so far, and report the 598-bp cDNA sequence, the chromosomal localization and the tissue expression of an additional subunit, the B17 subunit. The cDNA open reading frame of B17 comprises 387 bp and encodes a protein of 128 amino acids (calculated M r 15.5 kDa). There is 82.7% and 78.1% homology, respectively, at the cDNA and amino acid level with the bovine counterpart. The gene of the B17 subunit has been mapped to chromosome 2. Multiple-tissue dot-blots showed ubiquitous expression of the mRNA with relatively higher expression in tissues known for their high energy demand. Of these, kidney showed the highest expression. Mutational analysis of the subunit revealed no mutations or polymorphisms in 20 patients with isolated enzymatic complex I deficiency in cultured skin fibroblasts.
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