[HTML][HTML] The lipidation by hepatocytes of human apolipoprotein AI occurs by both ABCA1-dependent and-independent pathways
RS Kiss, DC McManus, V Franklin, WL Tan… - Journal of Biological …, 2003 - ASBMB
The pathways of hepatic intra-and peri-cellular lipidation of apolipoprotein AI (apoA-I) were
studied by infecting primary mouse hepatocytes from either apoA-I-deficient or ABCA1-
deficient mice with a recombinant adenovirus expressing the human apoA-I (hapoA-I) cDNA
(endo apoA-I) or incubating the hepatocytes with exogenously added hapoA-I (exo apoA-I)
and examining the hapoA-I-containing lipoproteins formed. The cells, maintained in serum-
free medium, were labeled with [3 H] choline, and the cell medium was separated by fast …
studied by infecting primary mouse hepatocytes from either apoA-I-deficient or ABCA1-
deficient mice with a recombinant adenovirus expressing the human apoA-I (hapoA-I) cDNA
(endo apoA-I) or incubating the hepatocytes with exogenously added hapoA-I (exo apoA-I)
and examining the hapoA-I-containing lipoproteins formed. The cells, maintained in serum-
free medium, were labeled with [3 H] choline, and the cell medium was separated by fast …