[HTML][HTML] Biosynthesis of ganglioside mimics in Campylobacter jejuni OH4384: identification of the glycosyltransferase genes, enzymatic synthesis of model …
M Gilbert, JR Brisson, MF Karwaski… - Journal of Biological …, 2000 - ASBMB
We have applied two strategies for the cloning of four genes responsible for the biosynthesis
of the GT1a ganglioside mimic in the lipooligosaccharide (LOS) of a bacterial pathogen,
Campylobacter jejuni OH4384, which has been associated with Guillain-Barré syndrome.
We first cloned a gene encoding an α-2, 3-sialyltransferase (cst-I) using an activity screening
strategy. We then used nucleotide sequence information from the recently completed
sequence from C. jejuni NCTC 11168 to amplify a region involved in LOS biosynthesis from …
of the GT1a ganglioside mimic in the lipooligosaccharide (LOS) of a bacterial pathogen,
Campylobacter jejuni OH4384, which has been associated with Guillain-Barré syndrome.
We first cloned a gene encoding an α-2, 3-sialyltransferase (cst-I) using an activity screening
strategy. We then used nucleotide sequence information from the recently completed
sequence from C. jejuni NCTC 11168 to amplify a region involved in LOS biosynthesis from …