[HTML][HTML] A cell-free assay for detecting HDL that is dysfunctional in preventing the formation of or inactivating oxidized phospholipids
M Navab, SY Hama, GP Hough… - Journal of lipid …, 2001 - ASBMB
We have developed a novel and rapid cell-free assay of the ability of HDL to prevent the
formation of or inactivate oxidized phospholipids. HDL was tested for its ability to inhibit the
oxidation of LDL, or inhibit the oxidation of l-α-1-palmitoyl-2-arachidonoyl-sn-glycero-3-
phosphorylcholine (PAPC) by hydroperoxyoctadecadienoic acid (HPODE), or inactivate
oxidized PAPC (Ox-PAPC). In each case the fluorescent signal generated in the presence of
the test substances and the test HDL was determined. As little as 2.5 μg of normal human …
formation of or inactivate oxidized phospholipids. HDL was tested for its ability to inhibit the
oxidation of LDL, or inhibit the oxidation of l-α-1-palmitoyl-2-arachidonoyl-sn-glycero-3-
phosphorylcholine (PAPC) by hydroperoxyoctadecadienoic acid (HPODE), or inactivate
oxidized PAPC (Ox-PAPC). In each case the fluorescent signal generated in the presence of
the test substances and the test HDL was determined. As little as 2.5 μg of normal human …