Reduced ob mRNA in hypophysectomised rats is not restored by growth hormone (GH), but further suppressed by exogenously administered insulin-like growth factor …
M Böni-Schnetzler, MA Gosteli-Peter, W Moritz… - Biochemical and …, 1996 - Elsevier
M Böni-Schnetzler, MA Gosteli-Peter, W Moritz, ER Froesch, J Zapf
Biochemical and biophysical research communications, 1996•ElsevierTo examine whether GH and IGF-I participate in the regulation of obese (ob) mRNA
expression we determined ob mRNA levels in epididydimal fat pads of hypophysectomised
(hypox) rats, hypox rats treated with recombinant human (rh) GH or rhIGF-I and normal,
weight-matched controls. We found that 1. ob mRNA was markedly suppressed after
hypophysectomy (37±25% of controls), 2. GH infusion had no effect on ob mRNA, but
stimulated IGF-I mRNA in fat pads, 3. IGF-I treatment further suppressed ob mRNA …
expression we determined ob mRNA levels in epididydimal fat pads of hypophysectomised
(hypox) rats, hypox rats treated with recombinant human (rh) GH or rhIGF-I and normal,
weight-matched controls. We found that 1. ob mRNA was markedly suppressed after
hypophysectomy (37±25% of controls), 2. GH infusion had no effect on ob mRNA, but
stimulated IGF-I mRNA in fat pads, 3. IGF-I treatment further suppressed ob mRNA …
To examine whether GH and IGF-I participate in the regulation of obese (ob) mRNA expression we determined ob mRNA levels in epididydimal fat pads of hypophysectomised (hypox) rats, hypox rats treated with recombinant human (rh) GH or rhIGF-I and normal, weight-matched controls. We found that 1. ob mRNA was markedly suppressed after hypophysectomy (37 ± 25% of controls), 2. GH infusion had no effect on ob mRNA, but stimulated IGF-I mRNA in fat pads, 3. IGF-I treatment further suppressed ob mRNA (3.5% ± 0.6% of controls) and 4. serum insulin levels were decreased in all hypox groups (11.2 to 15.9% of controls). In conclusion, exogenous and GH-induced IGF-I differ in their effects on ob mRNA expression and GH is unable to restore ob mRNA towards normal at low insulin levels.
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