[21] Thioredoxin and thioredoxin reductase
A Holmgren, M Bjornstedt - Methods in enzymology, 1995 - Elsevier
Publisher Summary This chapter summarizes current methods to determine Trx and
thioredoxin reductase (TR). Thioredoxin (Trx) is a small (Mr 12,000) multifunctional and
ubiquitous protein characterized by having a redox-active disulfide/dithiol within the
conserved active site sequence:-Trp-Cys-Gly-Pro-Cys-. Oxidized thioredoxin (Trx-S 2) has a
disulfide, and reduced thioredoxin [Trx-(SH) 2] has a dithiol. Thioredoxin reductase
specifically reduces Trx-S 2 to Trx-(SH) 2 using NADPH. The Trx-(SH) 2 form is a powerful …
thioredoxin reductase (TR). Thioredoxin (Trx) is a small (Mr 12,000) multifunctional and
ubiquitous protein characterized by having a redox-active disulfide/dithiol within the
conserved active site sequence:-Trp-Cys-Gly-Pro-Cys-. Oxidized thioredoxin (Trx-S 2) has a
disulfide, and reduced thioredoxin [Trx-(SH) 2] has a dithiol. Thioredoxin reductase
specifically reduces Trx-S 2 to Trx-(SH) 2 using NADPH. The Trx-(SH) 2 form is a powerful …