Developmental regulation of D-β-hydroxybutyrate dehydrogenase in rat liver and brain
WW Zhang, S Churchill, P Churchill - FEBS letters, 1989 - Elsevier
WW Zhang, S Churchill, P Churchill
FEBS letters, 1989•ElsevierThe activities of ketone-metabolizing enzymes in rat brain increase 3-to 5-fold during the
suckling period before decreasing to the adult level after weaning. We have observed that a
similar developmental pattern also exists for D-β-hydroxybutyrate dehydrogenase (BDH) in
rat liver. Utilizing antibodies prepared against the purified protein we determined that the
changes in BDH activities in both brain and liver are due to changes in the amount of BDH in
the mitochondria. In vitro translations of isolated RNA followed by immunoprecipitation …
suckling period before decreasing to the adult level after weaning. We have observed that a
similar developmental pattern also exists for D-β-hydroxybutyrate dehydrogenase (BDH) in
rat liver. Utilizing antibodies prepared against the purified protein we determined that the
changes in BDH activities in both brain and liver are due to changes in the amount of BDH in
the mitochondria. In vitro translations of isolated RNA followed by immunoprecipitation …
Abstract
The activities of ketone-metabolizing enzymes in rat brain increase 3- to 5-fold during the suckling period before decreasing to the adult level after weaning. We have observed that a similar developmental pattern also exists for D-β-hydroxybutyrate dehydrogenase (BDH) in rat liver. Utilizing antibodies prepared against the purified protein we determined that the changes in BDH activities in both brain and liver are due to changes in the amount of BDH in the mitochondria. In vitro translations of isolated RNA followed by immunoprecipitation revealed that the increase in BDH activity and content was correlated with an increase in the level of functional BDH-mRNA in both liver and brain.
Hydroxybutyrate dehydrogenase, D-β-; Developmental regulation
Elsevier