Production of monoclonal antibodies: strategy and tactics
SFS Groth, D Scheidegger - Journal of immunological methods, 1980 - Elsevier
A myeloma line has been developed which produces no globulin chains of its own, has a
duplication time of 8.7 h, fuses effectively with B-lymphoblasts and produces stable hybrids.
An enhancing effect of macrophages on hybridoma yields has been observed. Among the
fusing agents tested, PEG of mol. wt. 4000 gave the best results, 20° C being the optimum
working temperature. The maintenance medium of choice has been found to be Iscove's
with 10% FCS. Direct exposure of fusion cultures to a selective medium with hypoxanthine …
duplication time of 8.7 h, fuses effectively with B-lymphoblasts and produces stable hybrids.
An enhancing effect of macrophages on hybridoma yields has been observed. Among the
fusing agents tested, PEG of mol. wt. 4000 gave the best results, 20° C being the optimum
working temperature. The maintenance medium of choice has been found to be Iscove's
with 10% FCS. Direct exposure of fusion cultures to a selective medium with hypoxanthine …
[CITATION][C] Production of monoclonal antibodies: strategy and tactics
A Fazekas - J Immunol Methods, 1980 - cir.nii.ac.jp