[HTML][HTML] Glucose generates sub-plasma membrane ATP microdomains in single islet β-cells: potential role for strategically located mitochondria
HJ Kennedy, AE Pouli, EK Ainscow, LS Jouaville… - Journal of Biological …, 1999 - ASBMB
Increases in the concentration of free ATP within the islet β-cell may couple elevations in
blood glucose to insulin release by closing ATP-sensitive K+(K ATP) channels and
activating Ca 2+ influx. Here, we use recombinant targeted luciferases and photon counting
imaging to monitor changes in free [ATP] in subdomains of single living MIN6 and primary β-
cells. Resting [ATP] in the cytosol ([ATP] c), in the mitochondrial matrix ([ATP] m), and
beneath the plasma membrane ([ATP] pm) were similar (∼ 1 mm). Elevations in …
blood glucose to insulin release by closing ATP-sensitive K+(K ATP) channels and
activating Ca 2+ influx. Here, we use recombinant targeted luciferases and photon counting
imaging to monitor changes in free [ATP] in subdomains of single living MIN6 and primary β-
cells. Resting [ATP] in the cytosol ([ATP] c), in the mitochondrial matrix ([ATP] m), and
beneath the plasma membrane ([ATP] pm) were similar (∼ 1 mm). Elevations in …