Biotin uptake in cultured hepatocytes from normal and biotin-deficient rats
D Weiner, B Wolf - Biochemical medicine and metabolic biology, 1990 - Elsevier
Biotin uptake was studied in isolated cultured hepatocytes of normal and biotin-deficient
rats. Biotin uptake was temperature-dependent with respect to physical, but not to chemical,
processes, proportional to the exogenous biotin concentration in the medium, independent
of pH and sodium ion concentrations of the medium, and uneffected by the presence of
structural analogues of biotin or metabolic inhibitors in both normal and biotin-deficient
hepatocytes. These results suggest that biotin uptake occurs by a passive, nonmediated …
rats. Biotin uptake was temperature-dependent with respect to physical, but not to chemical,
processes, proportional to the exogenous biotin concentration in the medium, independent
of pH and sodium ion concentrations of the medium, and uneffected by the presence of
structural analogues of biotin or metabolic inhibitors in both normal and biotin-deficient
hepatocytes. These results suggest that biotin uptake occurs by a passive, nonmediated …