[HTML][HTML] Characterization of rapid membrane internalization and recycling
M Hao, FR Maxfield - Journal of Biological Chemistry, 2000 - ASBMB
Lipids and other membrane constituents recycle between the plasma membrane and
intracellular endocytic compartments. In CHO cells, approximately half of the internalized C
6-NBD-SM, a fluorescent lipid analogue widely used as a membrane maker, recycles via the
endocytic recycling compartment with at 1 2 of∼ 12 min (Mayor, S., Presley, JF, and
Maxfield, FR (1993) J. Cell Biol. 121, 1257–1269). Surprisingly, the rest returns to the
plasma membrane very quickly. A detailed kinetic study presented in this paper indicates …
intracellular endocytic compartments. In CHO cells, approximately half of the internalized C
6-NBD-SM, a fluorescent lipid analogue widely used as a membrane maker, recycles via the
endocytic recycling compartment with at 1 2 of∼ 12 min (Mayor, S., Presley, JF, and
Maxfield, FR (1993) J. Cell Biol. 121, 1257–1269). Surprisingly, the rest returns to the
plasma membrane very quickly. A detailed kinetic study presented in this paper indicates …