In vitro release of growth hormone-releasing factor (GRF) from the hypothalamus: somatostatin inhibits GRF release
N Yamauchi, T Shibasaki, N Ling, H Demura - Regulatory peptides, 1991 - Elsevier
The manner of release of growth hormone-releasing factor (GRF) from the rat hypothalamus
was studied in a perifusion system using a highly sensitive radioimmunoassay for rat GRF.
The recovery of GRF in this system was 50–60%. The release of GRF from the rat
hypothalamic blocks was almost stable for 20–240 min after the start of the perifusion and
was stimulated by depolarization induced by high K+ concentration. The release of GRF was
inhibited by somatostatin at concentrations of 10− 11 to 10− 8 M with maximum inhibition to …
was studied in a perifusion system using a highly sensitive radioimmunoassay for rat GRF.
The recovery of GRF in this system was 50–60%. The release of GRF from the rat
hypothalamic blocks was almost stable for 20–240 min after the start of the perifusion and
was stimulated by depolarization induced by high K+ concentration. The release of GRF was
inhibited by somatostatin at concentrations of 10− 11 to 10− 8 M with maximum inhibition to …