Surface glucose transporters of intact muscles were photolabeled with the membrane impenneant ATB‐[2‐³H]BMPA reagent and localized by autoradiography. We found sparse labeling of the glucose transporters by ATB‐[2‐³H]BMPA on the sarcolemmal membrane around the muscle fiber. The majority of label was on the interior of the muscle fiber, at a discrete site which matched the distribution of AI junctions and which was presumed to be on the exterior surface of T‐tubules. The amount of photolabel on the T‐tubule was increased in response to insulin and wasblocked by cytochalasin B. These results support the concept that glucose transport may occur predominately across the T‐tubule membrane under basal and insulin‐stimulated conditions.