Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A
J. Clin. Invest. Utpal Pal, et al. 106:561 doi:10.1172/JCI9427 [Go to this article.]

Figure 7
Binding of OspAs with mutations within amino acids 85–103 or 229–247 to TGE. Site-directed mutagenesis was performed to generate OspAs, designated M1–M6 with substitution in amino acids that are conserved between B. burgdorferi species within region 85–103 and 229–247 (Figure 6d). M1–M6 contain the following amino acid replacements: M1 (KVK85–87 to ΔEVE), M2 (DL93–94 to ΔAR), M3 (EVFK100–103 to ΔAVFA), M4 (K231 to ΔA), M5 (VF236–237 to ΔGS), and M6 (TIT242–244 to ΔANA). Binding of OspA and M1–M6 to TGE is depicted. B represents binding of OspA to BSA (control). M1–M6 showed a similar degree of binding to BSA (data not shown). Bars represent the mean ± SD from four experiments. ^AP < 0.001.