Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A
J. Clin. Invest. Utpal Pal, et al. 106:561 doi:10.1172/JCI9427 [Go to this article.]

Figure 3
Effect of increasing OspA on OspA-TGE binding, and OspA-OspA binding. (a) OspA binding to TGE is not saturable. OD was measured at 5 minutes. (b) Effect of excess unlabeled OspA on the binding of labeled OspA to TGE. TGE was probed with FITC-labeled OspA and increasing amounts of unlabeled OspA. These data represent the mean ± SD from three experiments. OD values were measured at 15 minutes. (c) Binding of OspA with OspA. OspA from B. burgdorferi N40, B. burgdorferi 25015, or BSA was probed with FITC-labeled OspA (25015-F, black bars; or N40-F, gray bars). Bars represent the mean ± SD from three experiments. OD values were measured at 15 minutes.